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What are Columns?

Liquid and gas chromatography instruments wouldn't work without columns.

Both liquid and gas chromatography analyze samples by separating them into their individual chemical components. A column, a long tube lined with specialized chemicals, is required for this process as it forms part of the instrument which performs the separation. When a sample makes its way through the tube, certain components 'stick' to the inside of the tube, causing them to separate and flow more slowly through the column.

Chromatography columns

A column is a long tube, typically coiled as above, in chromatography instruments

Early columns used decades ago were made of glass, which was extremely fragile, or of steel, which could react with some analytes. In 1979, this changed as Hewlett-Packard introduced fused-silica capillary columns which were inert like glass but much less fragile. The fused-silica columns, due to their robustness and the results they were able to produce, paved the way for gas chromatography to be adopted as a mainstream laboratory technique.

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Jonathan Stokes - 16/04/2018 15:50
An informative article. Fused-silica capillary columns are used routinely in a wide variety of gas chromatography applications ranging from environmental analysis to food testing to pharmaceutical product quality assurance. 


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